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KMID : 1007520100190020479
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Food Science and Biotechnology
2010 Volume.19 No. 2 p.479 ~ p.486
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Macrophage Stimulating Polysaccharide Purified from Peels of Grape (Vitis labrusca)
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Kim Dae-Jung
Lee Jun-Soo
Kim Kee-Jong
Hong Ha-Cheol
Shin Kwang-Soon
Yu Kwang-Won
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Abstract
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To characterize the macrophage stimulating polysaccharide in grape (Vitis labrusca) peels, the active crude polysaccharide (VL-3) has been fractionated from the hot-water extract of grape peels. A macrophage stimulating polysaccharide-rich fraction (VL-3IIb-1-1) was purified from VL-3 by 3 successive column chromatographies on DEAE-Sepharose CL-6B, Sepharose CL-6B, and Sephacryl S-300. VL-3IIb-1-1 was eluted as a single peak on high performance liquid chromatography (HPLC) and its molecular weight was estimated to be 194 kDa. VL- 3IIb-1-1 consisted mainly of Ara and Gal in addition to uronic acid (GalA+GlcA) (molar ratio 1.00:0.81:0.72). Methylation analysis indicated that VL-3IIb-1-1 consisted mainly of terminal Araf, 4- or 5- linked Ara, 2,4-branched Rha, 6- or 3,4- or 3,6-branched Gal, and 3,4,6-branched Glc. Single radial gel diffusion also indicated that VL-3IIb- 1-1 showed an intermediate reactivity with β-glucosyl-Yariv antigen. In addition, oral administration of VL-3IIb enhanced the stimulatory responses of macrophage stimulating activity ex vivo. Therefore, VL-3IIb-1-1 purified from grape peels is suggested to be pectic polysaccharide with arabino-3,6- galactan, and it is assumed that VL-3IIb-1-1 plays an important role for expression of its activity.
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KEYWORD
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grape peel, macrophage stimulation, methylation analysis, β-glucosyl-Yariv antigen, pectic polysaccharide
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